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Image Search Results
Journal: LWT
Article Title: Structural identification and immunomodulatory effects of chicken egg white glycopeptides
doi: 10.1016/j.lwt.2024.116195
Figure Lengend Snippet: Fig. 3. (A) Effects on the viability of RAW264.7 cells stimulated by EWG (egg white glycopeptides) at different concentrations for 8 h, data are shown as means ± SEM (n = 3); (B) EWG induces TNF-α secretion. RAW264.7 cells were incubated with various samples of LPS (25 ng/mL), low, medium and high EWG (5, 25, 125 μg/ mL) and LPS + EWG (25 ng/mL LPS + 125 μg/mL EWG) for 8 h. The production of TNF-α in the culture supernatant was detected by ELISA. CK: control group; LPS: lipopolysaccharide; EWG: egg white glycopeptides. Data are shown as means ± SEM (n = 3). Different lowercase roman letters indicate significant differences between groups (p < 0.05).
Article Snippet: Tumor necrosis factor alpha (TNF-α) content was measured using the
Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control
Journal: Allergy, Asthma & Immunology Research
Article Title: The TL1A-DR3 Axis in Asthma: Membrane-Bound and Secreted TL1A Co-Determined the Development of Airway Remodeling
doi: 10.4168/aair.2022.14.2.233
Figure Lengend Snippet: (A and B) TL1A expression profile graph in GSE18965 and differential expression analysis between asthmatics patients and healthy controls. (C) Expression of TL1A by immunohistochemical collagen levels by Masson staining in controls and subjects with asthma. Original magnification ×400. (D) Quantification of TL1A staining and collagen volume fraction between 2 groups. (E and F ) Protein and mRNA expression of TL1A in lung tissue of mice 3 days after TL1A or PBS treatment. (G and H ) Immunofluorescence detection of TL1A protein. (I) Expression of soluble TL1A in induced sputum detected by ELISA. (H) Expression of soluble TL1A in BALF detected by ELISA in each group (n = 10). Data are expressed as mean ± standard deviation. TL1A, tumor necrosis factor ligand-related molecule 1A; PBS, phosphate buffered saline; BALF, bronchoalveolar lavage fluid. * P < 0.05, ** P < 0.01, *** P < 0.001 versus the corresponding group.
Article Snippet: The ELISA kits used were as follows:
Techniques: Expressing, Quantitative Proteomics, Immunohistochemical staining, Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Standard Deviation, Saline
Journal: Allergy, Asthma & Immunology Research
Article Title: The TL1A-DR3 Axis in Asthma: Membrane-Bound and Secreted TL1A Co-Determined the Development of Airway Remodeling
doi: 10.4168/aair.2022.14.2.233
Figure Lengend Snippet: (A-D) H&E and Masson staining were used to observe histopathological changes after TL1A treatment in the mouse model of asthma. Histopathological lesions were analyzed quantitatively. (E-G) Protein expression of fibrosis indicator (collagen I and α-SMA) after treatment and subsequent quantitative analysis. (H-I) Immunofluorescence detection of fibrosis “indicator” proteins and their quantitative analyses. (J) Expression of IL-4, IL-5, and IL-13 in the blood detected by ELISA. (K) AHR was measured by airway reactivity to increased doses of inhaled methacholine treatment. Administration of TL1A can increase methacholine-induced AHR. TL1A, tumor necrosis factor ligand-related molecule 1A; OVA, ovalbumin; H&E, hematoxylin and eosin; α-SMA, α-smooth muscle actin; IL, interleukin; AHR, airway hyper-responsiveness; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. * P < 0.05, ** P < 0.01.
Article Snippet: The ELISA kits used were as follows:
Techniques: Staining, Expressing, Immunofluorescence, Enzyme-linked Immunosorbent Assay